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rabbit anti cart antiserum  (Phoenix Pharmaceuticals)

 
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    Phoenix Pharmaceuticals rabbit anti cart antiserum
    Rabbit Anti Cart Antiserum, supplied by Phoenix Pharmaceuticals, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+anti-cart+antibody/anti+cart/pm41618728-136-0-3
    Average 86 stars, based on 1 article reviews
    rabbit anti cart antiserum - by Bioz Stars, 2026-10
    86/100 stars

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    Related Articles

    Expressing:

    Article Title: Immunoreactivity for cocaine- and amphetamine-regulated transcript in rat sympathetic preganglionic neurons projecting to sympathetic ganglia and the adrenal medulla.
    Article Snippet: Many sympathetic preganglionic neurons (SPN) in the intermediolateral cell column (IML) contain cocaineand amphetamine-regulated transcript (CART), but the function of these CART-immunoreactive (IR) neurons is unknown.. To test the possibility that CART might mark SPN involved in cardiovascular regulation, we first established whether all CART neurons in the spinal cord were SPN by double-immunofluorescent labelling for CART and choline acetyltransferase (ChAT).. All autonomic subnuclei contained SPN immunoreactive for ChAT plus CART.

    Article Title: Cocaine- and amphetamine-regulated transcript in catecholamine and noncatecholamine presympathetic vasomotor neurons of rat rostral ventrolateral medulla.
    Article Snippet: Presympathetic vasomotor adrenergic (C1) and nonadrenergic (non-C1) neurons in the rostral ventrolateral medulla (RVLM) provide the main excitatory drive to cardiovascular sympathetic preganglionic neurons in the spinal cord.. C1 and non-C1 neurons contain cocaineand amphetamine-regulated transcript (CART), suggesting that CART may be a common marker for RVLM presympathetic neurons.. To test this hypothesis, we first used doubleimmunofluorescence staining for CART and tyrosine hydroxylase (TH) to quantify CARTimmunoreactive (-IR) catecholamine and noncatecholamine neurons in the C1 region.

    Article Title: Wide-field Diffuse Amacrine Cells in the Monkey Retina Contain Immunoreactive Cocaine- and Amphetamine-Regulated Transcript (CART)
    Article Snippet: Briefly, antibody was concurrently subjected to two conditions for 45min at room temperature: (1) Preincubation of 1:5,000 rabbit anti-CART antibody (Phoenix Pharmaceuticals, Catalog No. H-003-62) with a 50-fold higher concentration of the antigen (CART (55-102)(Rat, Mouse, Bovine), Catalog No. 003-62, Phoenix Pharmaceuticals, Inc., Burlingame, CA) in 0.01M phosphate buffered saline (PBS, Sigma-Aldrich, Saint Louis, MO, Catalog No. P-3813) with 0.3% TritonTM X-100 (SIGMA-ALDRICH, Saint Louis, MO, Catalog No. X100), and (2) 1:5,000 rabbit anti-CART antibody (Phoenix Pharmaceuticals, Catalog No.H-003-62) alone in PBS with 0.3% TritonTM X-100.

    Article Title: Wide-field Diffuse Amacrine Cells in the Monkey Retina Contain Immunoreactive Cocaine- and Amphetamine-Regulated Transcript (CART)
    Article Snippet: The majority of experiments were done using the rabbit anti-CART antibody (Phoenix Pharmaceuticals, Inc., Burlingame, CA, Catalog No.H-003-62).

    Article Title: Electrophysiological properties of melanin-concentrating hormone neuron subpopulations defined by anatomical localization and CART expression
    Article Snippet: To identify co-localization of CART in MCH neurons, brain slices were treated with a cocktail of goat anti-MCH antibody (1:500; sc14509, Santa Cruz Biotechnology Inc.) and rabbit anti-CART antibody (1:500; H-003-62, Phoenix Pharmaceuticals Inc.), followed by appropriate secondary antibodies and AMCA-streptavidin.

    Membrane:

    Article Title: Immunoreactivity for cocaine- and amphetamine-regulated transcript in rat sympathetic preganglionic neurons projecting to sympathetic ganglia and the adrenal medulla.
    Article Snippet: Many sympathetic preganglionic neurons (SPN) in the intermediolateral cell column (IML) contain cocaineand amphetamine-regulated transcript (CART), but the function of these CART-immunoreactive (IR) neurons is unknown.. To test the possibility that CART might mark SPN involved in cardiovascular regulation, we first established whether all CART neurons in the spinal cord were SPN by double-immunofluorescent labelling for CART and choline acetyltransferase (ChAT).. All autonomic subnuclei contained SPN immunoreactive for ChAT plus CART.

    Article Title: Cocaine- and amphetamine-regulated transcript in catecholamine and noncatecholamine presympathetic vasomotor neurons of rat rostral ventrolateral medulla.
    Article Snippet: Presympathetic vasomotor adrenergic (C1) and nonadrenergic (non-C1) neurons in the rostral ventrolateral medulla (RVLM) provide the main excitatory drive to cardiovascular sympathetic preganglionic neurons in the spinal cord.. C1 and non-C1 neurons contain cocaineand amphetamine-regulated transcript (CART), suggesting that CART may be a common marker for RVLM presympathetic neurons.. To test this hypothesis, we first used doubleimmunofluorescence staining for CART and tyrosine hydroxylase (TH) to quantify CARTimmunoreactive (-IR) catecholamine and noncatecholamine neurons in the C1 region.

    Article Title: Wide-field Diffuse Amacrine Cells in the Monkey Retina Contain Immunoreactive Cocaine- and Amphetamine-Regulated Transcript (CART)
    Article Snippet: Briefly, antibody was concurrently subjected to two conditions for 45min at room temperature: (1) Preincubation of 1:5,000 rabbit anti-CART antibody (Phoenix Pharmaceuticals, Catalog No. H-003-62) with a 50-fold higher concentration of the antigen (CART (55-102)(Rat, Mouse, Bovine), Catalog No. 003-62, Phoenix Pharmaceuticals, Inc., Burlingame, CA) in 0.01M phosphate buffered saline (PBS, Sigma-Aldrich, Saint Louis, MO, Catalog No. P-3813) with 0.3% TritonTM X-100 (SIGMA-ALDRICH, Saint Louis, MO, Catalog No. X100), and (2) 1:5,000 rabbit anti-CART antibody (Phoenix Pharmaceuticals, Catalog No.H-003-62) alone in PBS with 0.3% TritonTM X-100.

    Article Title: Wide-field Diffuse Amacrine Cells in the Monkey Retina Contain Immunoreactive Cocaine- and Amphetamine-Regulated Transcript (CART)
    Article Snippet: The majority of experiments were done using the rabbit anti-CART antibody (Phoenix Pharmaceuticals, Inc., Burlingame, CA, Catalog No.H-003-62).

    Article Title: Electrophysiological properties of melanin-concentrating hormone neuron subpopulations defined by anatomical localization and CART expression
    Article Snippet: To identify co-localization of CART in MCH neurons, brain slices were treated with a cocktail of goat anti-MCH antibody (1:500; sc14509, Santa Cruz Biotechnology Inc.) and rabbit anti-CART antibody (1:500; H-003-62, Phoenix Pharmaceuticals Inc.), followed by appropriate secondary antibodies and AMCA-streptavidin.

    Comparison:

    Article Title: Immunoreactivity for cocaine- and amphetamine-regulated transcript in rat sympathetic preganglionic neurons projecting to sympathetic ganglia and the adrenal medulla.
    Article Snippet: Many sympathetic preganglionic neurons (SPN) in the intermediolateral cell column (IML) contain cocaineand amphetamine-regulated transcript (CART), but the function of these CART-immunoreactive (IR) neurons is unknown.. To test the possibility that CART might mark SPN involved in cardiovascular regulation, we first established whether all CART neurons in the spinal cord were SPN by double-immunofluorescent labelling for CART and choline acetyltransferase (ChAT).. All autonomic subnuclei contained SPN immunoreactive for ChAT plus CART.

    Article Title: Cocaine- and amphetamine-regulated transcript in catecholamine and noncatecholamine presympathetic vasomotor neurons of rat rostral ventrolateral medulla.
    Article Snippet: Presympathetic vasomotor adrenergic (C1) and nonadrenergic (non-C1) neurons in the rostral ventrolateral medulla (RVLM) provide the main excitatory drive to cardiovascular sympathetic preganglionic neurons in the spinal cord.. C1 and non-C1 neurons contain cocaineand amphetamine-regulated transcript (CART), suggesting that CART may be a common marker for RVLM presympathetic neurons.. To test this hypothesis, we first used doubleimmunofluorescence staining for CART and tyrosine hydroxylase (TH) to quantify CARTimmunoreactive (-IR) catecholamine and noncatecholamine neurons in the C1 region.

    Article Title: Wide-field Diffuse Amacrine Cells in the Monkey Retina Contain Immunoreactive Cocaine- and Amphetamine-Regulated Transcript (CART)
    Article Snippet: Briefly, antibody was concurrently subjected to two conditions for 45min at room temperature: (1) Preincubation of 1:5,000 rabbit anti-CART antibody (Phoenix Pharmaceuticals, Catalog No. H-003-62) with a 50-fold higher concentration of the antigen (CART (55-102)(Rat, Mouse, Bovine), Catalog No. 003-62, Phoenix Pharmaceuticals, Inc., Burlingame, CA) in 0.01M phosphate buffered saline (PBS, Sigma-Aldrich, Saint Louis, MO, Catalog No. P-3813) with 0.3% TritonTM X-100 (SIGMA-ALDRICH, Saint Louis, MO, Catalog No. X100), and (2) 1:5,000 rabbit anti-CART antibody (Phoenix Pharmaceuticals, Catalog No.H-003-62) alone in PBS with 0.3% TritonTM X-100.

    Article Title: Wide-field Diffuse Amacrine Cells in the Monkey Retina Contain Immunoreactive Cocaine- and Amphetamine-Regulated Transcript (CART)
    Article Snippet: The majority of experiments were done using the rabbit anti-CART antibody (Phoenix Pharmaceuticals, Inc., Burlingame, CA, Catalog No.H-003-62).

    Article Title: Electrophysiological properties of melanin-concentrating hormone neuron subpopulations defined by anatomical localization and CART expression
    Article Snippet: To identify co-localization of CART in MCH neurons, brain slices were treated with a cocktail of goat anti-MCH antibody (1:500; sc14509, Santa Cruz Biotechnology Inc.) and rabbit anti-CART antibody (1:500; H-003-62, Phoenix Pharmaceuticals Inc.), followed by appropriate secondary antibodies and AMCA-streptavidin.

    Injection:

    Article Title: Immunoreactivity for cocaine- and amphetamine-regulated transcript in rat sympathetic preganglionic neurons projecting to sympathetic ganglia and the adrenal medulla.
    Article Snippet: Many sympathetic preganglionic neurons (SPN) in the intermediolateral cell column (IML) contain cocaineand amphetamine-regulated transcript (CART), but the function of these CART-immunoreactive (IR) neurons is unknown.. To test the possibility that CART might mark SPN involved in cardiovascular regulation, we first established whether all CART neurons in the spinal cord were SPN by double-immunofluorescent labelling for CART and choline acetyltransferase (ChAT).. All autonomic subnuclei contained SPN immunoreactive for ChAT plus CART.

    Article Title: Cocaine- and amphetamine-regulated transcript in catecholamine and noncatecholamine presympathetic vasomotor neurons of rat rostral ventrolateral medulla.
    Article Snippet: Presympathetic vasomotor adrenergic (C1) and nonadrenergic (non-C1) neurons in the rostral ventrolateral medulla (RVLM) provide the main excitatory drive to cardiovascular sympathetic preganglionic neurons in the spinal cord.. C1 and non-C1 neurons contain cocaineand amphetamine-regulated transcript (CART), suggesting that CART may be a common marker for RVLM presympathetic neurons.. To test this hypothesis, we first used doubleimmunofluorescence staining for CART and tyrosine hydroxylase (TH) to quantify CARTimmunoreactive (-IR) catecholamine and noncatecholamine neurons in the C1 region.

    Article Title: Wide-field Diffuse Amacrine Cells in the Monkey Retina Contain Immunoreactive Cocaine- and Amphetamine-Regulated Transcript (CART)
    Article Snippet: Briefly, antibody was concurrently subjected to two conditions for 45min at room temperature: (1) Preincubation of 1:5,000 rabbit anti-CART antibody (Phoenix Pharmaceuticals, Catalog No. H-003-62) with a 50-fold higher concentration of the antigen (CART (55-102)(Rat, Mouse, Bovine), Catalog No. 003-62, Phoenix Pharmaceuticals, Inc., Burlingame, CA) in 0.01M phosphate buffered saline (PBS, Sigma-Aldrich, Saint Louis, MO, Catalog No. P-3813) with 0.3% TritonTM X-100 (SIGMA-ALDRICH, Saint Louis, MO, Catalog No. X100), and (2) 1:5,000 rabbit anti-CART antibody (Phoenix Pharmaceuticals, Catalog No.H-003-62) alone in PBS with 0.3% TritonTM X-100.

    Article Title: Wide-field Diffuse Amacrine Cells in the Monkey Retina Contain Immunoreactive Cocaine- and Amphetamine-Regulated Transcript (CART)
    Article Snippet: The majority of experiments were done using the rabbit anti-CART antibody (Phoenix Pharmaceuticals, Inc., Burlingame, CA, Catalog No.H-003-62).

    Article Title: Electrophysiological properties of melanin-concentrating hormone neuron subpopulations defined by anatomical localization and CART expression
    Article Snippet: To identify co-localization of CART in MCH neurons, brain slices were treated with a cocktail of goat anti-MCH antibody (1:500; sc14509, Santa Cruz Biotechnology Inc.) and rabbit anti-CART antibody (1:500; H-003-62, Phoenix Pharmaceuticals Inc.), followed by appropriate secondary antibodies and AMCA-streptavidin.

    Immunohistochemical staining:

    Article Title: Immunoreactivity for cocaine- and amphetamine-regulated transcript in rat sympathetic preganglionic neurons projecting to sympathetic ganglia and the adrenal medulla.
    Article Snippet: Many sympathetic preganglionic neurons (SPN) in the intermediolateral cell column (IML) contain cocaineand amphetamine-regulated transcript (CART), but the function of these CART-immunoreactive (IR) neurons is unknown.. To test the possibility that CART might mark SPN involved in cardiovascular regulation, we first established whether all CART neurons in the spinal cord were SPN by double-immunofluorescent labelling for CART and choline acetyltransferase (ChAT).. All autonomic subnuclei contained SPN immunoreactive for ChAT plus CART.

    Article Title: Cocaine- and amphetamine-regulated transcript in catecholamine and noncatecholamine presympathetic vasomotor neurons of rat rostral ventrolateral medulla.
    Article Snippet: Presympathetic vasomotor adrenergic (C1) and nonadrenergic (non-C1) neurons in the rostral ventrolateral medulla (RVLM) provide the main excitatory drive to cardiovascular sympathetic preganglionic neurons in the spinal cord.. C1 and non-C1 neurons contain cocaineand amphetamine-regulated transcript (CART), suggesting that CART may be a common marker for RVLM presympathetic neurons.. To test this hypothesis, we first used doubleimmunofluorescence staining for CART and tyrosine hydroxylase (TH) to quantify CARTimmunoreactive (-IR) catecholamine and noncatecholamine neurons in the C1 region.

    Article Title: Wide-field Diffuse Amacrine Cells in the Monkey Retina Contain Immunoreactive Cocaine- and Amphetamine-Regulated Transcript (CART)
    Article Snippet: Briefly, antibody was concurrently subjected to two conditions for 45min at room temperature: (1) Preincubation of 1:5,000 rabbit anti-CART antibody (Phoenix Pharmaceuticals, Catalog No. H-003-62) with a 50-fold higher concentration of the antigen (CART (55-102)(Rat, Mouse, Bovine), Catalog No. 003-62, Phoenix Pharmaceuticals, Inc., Burlingame, CA) in 0.01M phosphate buffered saline (PBS, Sigma-Aldrich, Saint Louis, MO, Catalog No. P-3813) with 0.3% TritonTM X-100 (SIGMA-ALDRICH, Saint Louis, MO, Catalog No. X100), and (2) 1:5,000 rabbit anti-CART antibody (Phoenix Pharmaceuticals, Catalog No.H-003-62) alone in PBS with 0.3% TritonTM X-100.

    Article Title: Wide-field Diffuse Amacrine Cells in the Monkey Retina Contain Immunoreactive Cocaine- and Amphetamine-Regulated Transcript (CART)
    Article Snippet: The majority of experiments were done using the rabbit anti-CART antibody (Phoenix Pharmaceuticals, Inc., Burlingame, CA, Catalog No.H-003-62).

    Article Title: Electrophysiological properties of melanin-concentrating hormone neuron subpopulations defined by anatomical localization and CART expression
    Article Snippet: To identify co-localization of CART in MCH neurons, brain slices were treated with a cocktail of goat anti-MCH antibody (1:500; sc14509, Santa Cruz Biotechnology Inc.) and rabbit anti-CART antibody (1:500; H-003-62, Phoenix Pharmaceuticals Inc.), followed by appropriate secondary antibodies and AMCA-streptavidin.

    Immunofluorescence:

    Article Title: Immunoreactivity for cocaine- and amphetamine-regulated transcript in rat sympathetic preganglionic neurons projecting to sympathetic ganglia and the adrenal medulla.
    Article Snippet: Many sympathetic preganglionic neurons (SPN) in the intermediolateral cell column (IML) contain cocaineand amphetamine-regulated transcript (CART), but the function of these CART-immunoreactive (IR) neurons is unknown.. To test the possibility that CART might mark SPN involved in cardiovascular regulation, we first established whether all CART neurons in the spinal cord were SPN by double-immunofluorescent labelling for CART and choline acetyltransferase (ChAT).. All autonomic subnuclei contained SPN immunoreactive for ChAT plus CART.

    Article Title: Cocaine- and amphetamine-regulated transcript in catecholamine and noncatecholamine presympathetic vasomotor neurons of rat rostral ventrolateral medulla.
    Article Snippet: Presympathetic vasomotor adrenergic (C1) and nonadrenergic (non-C1) neurons in the rostral ventrolateral medulla (RVLM) provide the main excitatory drive to cardiovascular sympathetic preganglionic neurons in the spinal cord.. C1 and non-C1 neurons contain cocaineand amphetamine-regulated transcript (CART), suggesting that CART may be a common marker for RVLM presympathetic neurons.. To test this hypothesis, we first used doubleimmunofluorescence staining for CART and tyrosine hydroxylase (TH) to quantify CARTimmunoreactive (-IR) catecholamine and noncatecholamine neurons in the C1 region.

    Article Title: Wide-field Diffuse Amacrine Cells in the Monkey Retina Contain Immunoreactive Cocaine- and Amphetamine-Regulated Transcript (CART)
    Article Snippet: Briefly, antibody was concurrently subjected to two conditions for 45min at room temperature: (1) Preincubation of 1:5,000 rabbit anti-CART antibody (Phoenix Pharmaceuticals, Catalog No. H-003-62) with a 50-fold higher concentration of the antigen (CART (55-102)(Rat, Mouse, Bovine), Catalog No. 003-62, Phoenix Pharmaceuticals, Inc., Burlingame, CA) in 0.01M phosphate buffered saline (PBS, Sigma-Aldrich, Saint Louis, MO, Catalog No. P-3813) with 0.3% TritonTM X-100 (SIGMA-ALDRICH, Saint Louis, MO, Catalog No. X100), and (2) 1:5,000 rabbit anti-CART antibody (Phoenix Pharmaceuticals, Catalog No.H-003-62) alone in PBS with 0.3% TritonTM X-100.

    Article Title: Wide-field Diffuse Amacrine Cells in the Monkey Retina Contain Immunoreactive Cocaine- and Amphetamine-Regulated Transcript (CART)
    Article Snippet: The majority of experiments were done using the rabbit anti-CART antibody (Phoenix Pharmaceuticals, Inc., Burlingame, CA, Catalog No.H-003-62).

    Article Title: Electrophysiological properties of melanin-concentrating hormone neuron subpopulations defined by anatomical localization and CART expression
    Article Snippet: To identify co-localization of CART in MCH neurons, brain slices were treated with a cocktail of goat anti-MCH antibody (1:500; sc14509, Santa Cruz Biotechnology Inc.) and rabbit anti-CART antibody (1:500; H-003-62, Phoenix Pharmaceuticals Inc.), followed by appropriate secondary antibodies and AMCA-streptavidin.

    Patch Clamp:

    Article Title: Immunoreactivity for cocaine- and amphetamine-regulated transcript in rat sympathetic preganglionic neurons projecting to sympathetic ganglia and the adrenal medulla.
    Article Snippet: Many sympathetic preganglionic neurons (SPN) in the intermediolateral cell column (IML) contain cocaineand amphetamine-regulated transcript (CART), but the function of these CART-immunoreactive (IR) neurons is unknown.. To test the possibility that CART might mark SPN involved in cardiovascular regulation, we first established whether all CART neurons in the spinal cord were SPN by double-immunofluorescent labelling for CART and choline acetyltransferase (ChAT).. All autonomic subnuclei contained SPN immunoreactive for ChAT plus CART.

    Article Title: Cocaine- and amphetamine-regulated transcript in catecholamine and noncatecholamine presympathetic vasomotor neurons of rat rostral ventrolateral medulla.
    Article Snippet: Presympathetic vasomotor adrenergic (C1) and nonadrenergic (non-C1) neurons in the rostral ventrolateral medulla (RVLM) provide the main excitatory drive to cardiovascular sympathetic preganglionic neurons in the spinal cord.. C1 and non-C1 neurons contain cocaineand amphetamine-regulated transcript (CART), suggesting that CART may be a common marker for RVLM presympathetic neurons.. To test this hypothesis, we first used doubleimmunofluorescence staining for CART and tyrosine hydroxylase (TH) to quantify CARTimmunoreactive (-IR) catecholamine and noncatecholamine neurons in the C1 region.

    Article Title: Wide-field Diffuse Amacrine Cells in the Monkey Retina Contain Immunoreactive Cocaine- and Amphetamine-Regulated Transcript (CART)
    Article Snippet: Briefly, antibody was concurrently subjected to two conditions for 45min at room temperature: (1) Preincubation of 1:5,000 rabbit anti-CART antibody (Phoenix Pharmaceuticals, Catalog No. H-003-62) with a 50-fold higher concentration of the antigen (CART (55-102)(Rat, Mouse, Bovine), Catalog No. 003-62, Phoenix Pharmaceuticals, Inc., Burlingame, CA) in 0.01M phosphate buffered saline (PBS, Sigma-Aldrich, Saint Louis, MO, Catalog No. P-3813) with 0.3% TritonTM X-100 (SIGMA-ALDRICH, Saint Louis, MO, Catalog No. X100), and (2) 1:5,000 rabbit anti-CART antibody (Phoenix Pharmaceuticals, Catalog No.H-003-62) alone in PBS with 0.3% TritonTM X-100.

    Article Title: Wide-field Diffuse Amacrine Cells in the Monkey Retina Contain Immunoreactive Cocaine- and Amphetamine-Regulated Transcript (CART)
    Article Snippet: The majority of experiments were done using the rabbit anti-CART antibody (Phoenix Pharmaceuticals, Inc., Burlingame, CA, Catalog No.H-003-62).

    Article Title: Electrophysiological properties of melanin-concentrating hormone neuron subpopulations defined by anatomical localization and CART expression
    Article Snippet: To identify co-localization of CART in MCH neurons, brain slices were treated with a cocktail of goat anti-MCH antibody (1:500; sc14509, Santa Cruz Biotechnology Inc.) and rabbit anti-CART antibody (1:500; H-003-62, Phoenix Pharmaceuticals Inc.), followed by appropriate secondary antibodies and AMCA-streptavidin.

    Concentration Assay:

    Article Title: Immunoreactivity for cocaine- and amphetamine-regulated transcript in rat sympathetic preganglionic neurons projecting to sympathetic ganglia and the adrenal medulla.
    Article Snippet: Many sympathetic preganglionic neurons (SPN) in the intermediolateral cell column (IML) contain cocaineand amphetamine-regulated transcript (CART), but the function of these CART-immunoreactive (IR) neurons is unknown.. To test the possibility that CART might mark SPN involved in cardiovascular regulation, we first established whether all CART neurons in the spinal cord were SPN by double-immunofluorescent labelling for CART and choline acetyltransferase (ChAT).. All autonomic subnuclei contained SPN immunoreactive for ChAT plus CART.

    Article Title: Cocaine- and amphetamine-regulated transcript in catecholamine and noncatecholamine presympathetic vasomotor neurons of rat rostral ventrolateral medulla.
    Article Snippet: Presympathetic vasomotor adrenergic (C1) and nonadrenergic (non-C1) neurons in the rostral ventrolateral medulla (RVLM) provide the main excitatory drive to cardiovascular sympathetic preganglionic neurons in the spinal cord.. C1 and non-C1 neurons contain cocaineand amphetamine-regulated transcript (CART), suggesting that CART may be a common marker for RVLM presympathetic neurons.. To test this hypothesis, we first used doubleimmunofluorescence staining for CART and tyrosine hydroxylase (TH) to quantify CARTimmunoreactive (-IR) catecholamine and noncatecholamine neurons in the C1 region.

    Article Title: Wide-field Diffuse Amacrine Cells in the Monkey Retina Contain Immunoreactive Cocaine- and Amphetamine-Regulated Transcript (CART)
    Article Snippet: Briefly, antibody was concurrently subjected to two conditions for 45min at room temperature: (1) Preincubation of 1:5,000 rabbit anti-CART antibody (Phoenix Pharmaceuticals, Catalog No. H-003-62) with a 50-fold higher concentration of the antigen (CART (55-102)(Rat, Mouse, Bovine), Catalog No. 003-62, Phoenix Pharmaceuticals, Inc., Burlingame, CA) in 0.01M phosphate buffered saline (PBS, Sigma-Aldrich, Saint Louis, MO, Catalog No. P-3813) with 0.3% TritonTM X-100 (SIGMA-ALDRICH, Saint Louis, MO, Catalog No. X100), and (2) 1:5,000 rabbit anti-CART antibody (Phoenix Pharmaceuticals, Catalog No.H-003-62) alone in PBS with 0.3% TritonTM X-100.

    Article Title: Wide-field Diffuse Amacrine Cells in the Monkey Retina Contain Immunoreactive Cocaine- and Amphetamine-Regulated Transcript (CART)
    Article Snippet: The majority of experiments were done using the rabbit anti-CART antibody (Phoenix Pharmaceuticals, Inc., Burlingame, CA, Catalog No.H-003-62).

    Article Title: Electrophysiological properties of melanin-concentrating hormone neuron subpopulations defined by anatomical localization and CART expression
    Article Snippet: To identify co-localization of CART in MCH neurons, brain slices were treated with a cocktail of goat anti-MCH antibody (1:500; sc14509, Santa Cruz Biotechnology Inc.) and rabbit anti-CART antibody (1:500; H-003-62, Phoenix Pharmaceuticals Inc.), followed by appropriate secondary antibodies and AMCA-streptavidin.

    Saline:

    Article Title: Immunoreactivity for cocaine- and amphetamine-regulated transcript in rat sympathetic preganglionic neurons projecting to sympathetic ganglia and the adrenal medulla.
    Article Snippet: Many sympathetic preganglionic neurons (SPN) in the intermediolateral cell column (IML) contain cocaineand amphetamine-regulated transcript (CART), but the function of these CART-immunoreactive (IR) neurons is unknown.. To test the possibility that CART might mark SPN involved in cardiovascular regulation, we first established whether all CART neurons in the spinal cord were SPN by double-immunofluorescent labelling for CART and choline acetyltransferase (ChAT).. All autonomic subnuclei contained SPN immunoreactive for ChAT plus CART.

    Article Title: Cocaine- and amphetamine-regulated transcript in catecholamine and noncatecholamine presympathetic vasomotor neurons of rat rostral ventrolateral medulla.
    Article Snippet: Presympathetic vasomotor adrenergic (C1) and nonadrenergic (non-C1) neurons in the rostral ventrolateral medulla (RVLM) provide the main excitatory drive to cardiovascular sympathetic preganglionic neurons in the spinal cord.. C1 and non-C1 neurons contain cocaineand amphetamine-regulated transcript (CART), suggesting that CART may be a common marker for RVLM presympathetic neurons.. To test this hypothesis, we first used doubleimmunofluorescence staining for CART and tyrosine hydroxylase (TH) to quantify CARTimmunoreactive (-IR) catecholamine and noncatecholamine neurons in the C1 region.

    Article Title: Wide-field Diffuse Amacrine Cells in the Monkey Retina Contain Immunoreactive Cocaine- and Amphetamine-Regulated Transcript (CART)
    Article Snippet: Briefly, antibody was concurrently subjected to two conditions for 45min at room temperature: (1) Preincubation of 1:5,000 rabbit anti-CART antibody (Phoenix Pharmaceuticals, Catalog No. H-003-62) with a 50-fold higher concentration of the antigen (CART (55-102)(Rat, Mouse, Bovine), Catalog No. 003-62, Phoenix Pharmaceuticals, Inc., Burlingame, CA) in 0.01M phosphate buffered saline (PBS, Sigma-Aldrich, Saint Louis, MO, Catalog No. P-3813) with 0.3% TritonTM X-100 (SIGMA-ALDRICH, Saint Louis, MO, Catalog No. X100), and (2) 1:5,000 rabbit anti-CART antibody (Phoenix Pharmaceuticals, Catalog No.H-003-62) alone in PBS with 0.3% TritonTM X-100.

    Article Title: Wide-field Diffuse Amacrine Cells in the Monkey Retina Contain Immunoreactive Cocaine- and Amphetamine-Regulated Transcript (CART)
    Article Snippet: The majority of experiments were done using the rabbit anti-CART antibody (Phoenix Pharmaceuticals, Inc., Burlingame, CA, Catalog No.H-003-62).

    Article Title: Electrophysiological properties of melanin-concentrating hormone neuron subpopulations defined by anatomical localization and CART expression
    Article Snippet: To identify co-localization of CART in MCH neurons, brain slices were treated with a cocktail of goat anti-MCH antibody (1:500; sc14509, Santa Cruz Biotechnology Inc.) and rabbit anti-CART antibody (1:500; H-003-62, Phoenix Pharmaceuticals Inc.), followed by appropriate secondary antibodies and AMCA-streptavidin.

    Incubation:

    Article Title: Immunoreactivity for cocaine- and amphetamine-regulated transcript in rat sympathetic preganglionic neurons projecting to sympathetic ganglia and the adrenal medulla.
    Article Snippet: Many sympathetic preganglionic neurons (SPN) in the intermediolateral cell column (IML) contain cocaineand amphetamine-regulated transcript (CART), but the function of these CART-immunoreactive (IR) neurons is unknown.. To test the possibility that CART might mark SPN involved in cardiovascular regulation, we first established whether all CART neurons in the spinal cord were SPN by double-immunofluorescent labelling for CART and choline acetyltransferase (ChAT).. All autonomic subnuclei contained SPN immunoreactive for ChAT plus CART.

    Article Title: Cocaine- and amphetamine-regulated transcript in catecholamine and noncatecholamine presympathetic vasomotor neurons of rat rostral ventrolateral medulla.
    Article Snippet: Presympathetic vasomotor adrenergic (C1) and nonadrenergic (non-C1) neurons in the rostral ventrolateral medulla (RVLM) provide the main excitatory drive to cardiovascular sympathetic preganglionic neurons in the spinal cord.. C1 and non-C1 neurons contain cocaineand amphetamine-regulated transcript (CART), suggesting that CART may be a common marker for RVLM presympathetic neurons.. To test this hypothesis, we first used doubleimmunofluorescence staining for CART and tyrosine hydroxylase (TH) to quantify CARTimmunoreactive (-IR) catecholamine and noncatecholamine neurons in the C1 region.

    Article Title: Wide-field Diffuse Amacrine Cells in the Monkey Retina Contain Immunoreactive Cocaine- and Amphetamine-Regulated Transcript (CART)
    Article Snippet: Briefly, antibody was concurrently subjected to two conditions for 45min at room temperature: (1) Preincubation of 1:5,000 rabbit anti-CART antibody (Phoenix Pharmaceuticals, Catalog No. H-003-62) with a 50-fold higher concentration of the antigen (CART (55-102)(Rat, Mouse, Bovine), Catalog No. 003-62, Phoenix Pharmaceuticals, Inc., Burlingame, CA) in 0.01M phosphate buffered saline (PBS, Sigma-Aldrich, Saint Louis, MO, Catalog No. P-3813) with 0.3% TritonTM X-100 (SIGMA-ALDRICH, Saint Louis, MO, Catalog No. X100), and (2) 1:5,000 rabbit anti-CART antibody (Phoenix Pharmaceuticals, Catalog No.H-003-62) alone in PBS with 0.3% TritonTM X-100.

    Article Title: Wide-field Diffuse Amacrine Cells in the Monkey Retina Contain Immunoreactive Cocaine- and Amphetamine-Regulated Transcript (CART)
    Article Snippet: The majority of experiments were done using the rabbit anti-CART antibody (Phoenix Pharmaceuticals, Inc., Burlingame, CA, Catalog No.H-003-62).

    Article Title: Electrophysiological properties of melanin-concentrating hormone neuron subpopulations defined by anatomical localization and CART expression
    Article Snippet: To identify co-localization of CART in MCH neurons, brain slices were treated with a cocktail of goat anti-MCH antibody (1:500; sc14509, Santa Cruz Biotechnology Inc.) and rabbit anti-CART antibody (1:500; H-003-62, Phoenix Pharmaceuticals Inc.), followed by appropriate secondary antibodies and AMCA-streptavidin.

    Affinity Purification:

    Article Title: Immunoreactivity for cocaine- and amphetamine-regulated transcript in rat sympathetic preganglionic neurons projecting to sympathetic ganglia and the adrenal medulla.
    Article Snippet: Many sympathetic preganglionic neurons (SPN) in the intermediolateral cell column (IML) contain cocaineand amphetamine-regulated transcript (CART), but the function of these CART-immunoreactive (IR) neurons is unknown.. To test the possibility that CART might mark SPN involved in cardiovascular regulation, we first established whether all CART neurons in the spinal cord were SPN by double-immunofluorescent labelling for CART and choline acetyltransferase (ChAT).. All autonomic subnuclei contained SPN immunoreactive for ChAT plus CART.

    Article Title: Cocaine- and amphetamine-regulated transcript in catecholamine and noncatecholamine presympathetic vasomotor neurons of rat rostral ventrolateral medulla.
    Article Snippet: Presympathetic vasomotor adrenergic (C1) and nonadrenergic (non-C1) neurons in the rostral ventrolateral medulla (RVLM) provide the main excitatory drive to cardiovascular sympathetic preganglionic neurons in the spinal cord.. C1 and non-C1 neurons contain cocaineand amphetamine-regulated transcript (CART), suggesting that CART may be a common marker for RVLM presympathetic neurons.. To test this hypothesis, we first used doubleimmunofluorescence staining for CART and tyrosine hydroxylase (TH) to quantify CARTimmunoreactive (-IR) catecholamine and noncatecholamine neurons in the C1 region.

    Article Title: Wide-field Diffuse Amacrine Cells in the Monkey Retina Contain Immunoreactive Cocaine- and Amphetamine-Regulated Transcript (CART)
    Article Snippet: Briefly, antibody was concurrently subjected to two conditions for 45min at room temperature: (1) Preincubation of 1:5,000 rabbit anti-CART antibody (Phoenix Pharmaceuticals, Catalog No. H-003-62) with a 50-fold higher concentration of the antigen (CART (55-102)(Rat, Mouse, Bovine), Catalog No. 003-62, Phoenix Pharmaceuticals, Inc., Burlingame, CA) in 0.01M phosphate buffered saline (PBS, Sigma-Aldrich, Saint Louis, MO, Catalog No. P-3813) with 0.3% TritonTM X-100 (SIGMA-ALDRICH, Saint Louis, MO, Catalog No. X100), and (2) 1:5,000 rabbit anti-CART antibody (Phoenix Pharmaceuticals, Catalog No.H-003-62) alone in PBS with 0.3% TritonTM X-100.

    Article Title: Wide-field Diffuse Amacrine Cells in the Monkey Retina Contain Immunoreactive Cocaine- and Amphetamine-Regulated Transcript (CART)
    Article Snippet: The majority of experiments were done using the rabbit anti-CART antibody (Phoenix Pharmaceuticals, Inc., Burlingame, CA, Catalog No.H-003-62).

    Article Title: Electrophysiological properties of melanin-concentrating hormone neuron subpopulations defined by anatomical localization and CART expression
    Article Snippet: To identify co-localization of CART in MCH neurons, brain slices were treated with a cocktail of goat anti-MCH antibody (1:500; sc14509, Santa Cruz Biotechnology Inc.) and rabbit anti-CART antibody (1:500; H-003-62, Phoenix Pharmaceuticals Inc.), followed by appropriate secondary antibodies and AMCA-streptavidin.



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    Image Search Results


    Electrophysiological characteristics of MCH neurons with or without CART expression. (Ai,Aii) Representative current clamp recording of CART-negative and -positive MCH neurons in different brain regions in male (Ai) and female mice (Aii) . (B–E) MCH neurons are grouped according to the CART expression and anatomical localization: MCH/CART− cells in LH (LH CART−) and MCH/CART+ cells in LH, ZI and MH. Graphs show passive membrane properties including Cm (B) , Rm (C) , time constant (D) and RMP (E) . (F,G) AP frequency (F) and latency to first AP (G) during 600-ms, 200-pA current injections. (H) Spike adaptation (ratio of first and last AP intervals). (I–L) Comparisons of AP waveform characteristics between male and female MCH neurons with or without CART expression, including AP threshold (I) , AP amplitude (J) , AHP amplitude (K) and half-width (L) . MCH/CART+ cells in all three anatomical areas are combined. $ p < 0.05, $$ p < 0.01, $$$ p < 0.001, $$$$ p < 0.0001, multiple comparisons between areas. ** p < 0.01, multiple comparison between individual groups. Mean ± SEM are shown.

    Journal: Frontiers in Cellular Neuroscience

    Article Title: Electrophysiological properties of melanin-concentrating hormone neuron subpopulations defined by anatomical localization and CART expression

    doi: 10.3389/fncel.2024.1439752

    Figure Lengend Snippet: Electrophysiological characteristics of MCH neurons with or without CART expression. (Ai,Aii) Representative current clamp recording of CART-negative and -positive MCH neurons in different brain regions in male (Ai) and female mice (Aii) . (B–E) MCH neurons are grouped according to the CART expression and anatomical localization: MCH/CART− cells in LH (LH CART−) and MCH/CART+ cells in LH, ZI and MH. Graphs show passive membrane properties including Cm (B) , Rm (C) , time constant (D) and RMP (E) . (F,G) AP frequency (F) and latency to first AP (G) during 600-ms, 200-pA current injections. (H) Spike adaptation (ratio of first and last AP intervals). (I–L) Comparisons of AP waveform characteristics between male and female MCH neurons with or without CART expression, including AP threshold (I) , AP amplitude (J) , AHP amplitude (K) and half-width (L) . MCH/CART+ cells in all three anatomical areas are combined. $ p < 0.05, $$ p < 0.01, $$$ p < 0.001, $$$$ p < 0.0001, multiple comparisons between areas. ** p < 0.01, multiple comparison between individual groups. Mean ± SEM are shown.

    Article Snippet: To identify co-localization of CART in MCH neurons, brain slices were treated with a cocktail of goat anti-MCH antibody (1:500; sc14509, Santa Cruz Biotechnology Inc.) and rabbit anti-CART antibody (1:500; H-003-62, Phoenix Pharmaceuticals Inc.), followed by appropriate secondary antibodies and AMCA-streptavidin.

    Techniques: Expressing, Membrane, Comparison

    CART-negative MCH neurons display H-currents. (A,B) X–Y scatter plot of H-current density recorded after the peak membrane potential reached in response to hyperpolarizing current steps applied to male (A) and female (B) MCH neurons. The current steps ranged from −200 to −50 pA. Values from individual cells are pooled according to cell type. (C,D) H-current density in response to a range of hyperpolarizing current injections in male (C) and female cells (D) . (E) A summary plot showing H-current density recorded from −200 pA injection. (F) Representative current clamp recording from a MCH/CART− cell before (baseline) and during ZD 7288 application. (G) Repeated measures of voltage sag at baseline and in the presence of ZD 7288 in MCH/CART− cells. (H) X–Y scatter plot of the membrane potential at the peak of a voltage sag and ensuing steady-state in MCH/CART− cells shown in (G) . (I) Repeated measures of voltage sag at baseline and in the presence of ZD 7288 in MCH/CART+ cells. (J) Example recording of depolarized and hyperpolarized CART-positive MCH neurons. A minor population of MCH/CART+ neurons shows depolarized RMP like that of CART− neurons (>−70 mV) but lacks H-current. (K) A summary graph showing that H-current is unique to MCH/CART− neurons. (L,M) Steady-state potential in response to 600-ms hyperpolarizing current steps of varying magnitude in male (L) and female (M) MCH neurons. (C–E) $$ p < 0.01, $$$ p < 0.001, $$$$ p < 0.0001, LH CART− vs. all CART+ groups. (G,K) ** p < 0.01, *** p < 0.001, **** p < 0.0001. (L,M) $ p < 0.05, $$$$ p < 0.0001, LH CART− vs. ZI; # p < 0.05, ## p < 0.01, LH CART− vs. LH CART+; & p < 0.05, &&& p < 0.001, LH CART− vs. MH. Mean ± SEM are shown. For (G–K) , male and female cells were pooled since no sex differences were found in H-current density (E) .

    Journal: Frontiers in Cellular Neuroscience

    Article Title: Electrophysiological properties of melanin-concentrating hormone neuron subpopulations defined by anatomical localization and CART expression

    doi: 10.3389/fncel.2024.1439752

    Figure Lengend Snippet: CART-negative MCH neurons display H-currents. (A,B) X–Y scatter plot of H-current density recorded after the peak membrane potential reached in response to hyperpolarizing current steps applied to male (A) and female (B) MCH neurons. The current steps ranged from −200 to −50 pA. Values from individual cells are pooled according to cell type. (C,D) H-current density in response to a range of hyperpolarizing current injections in male (C) and female cells (D) . (E) A summary plot showing H-current density recorded from −200 pA injection. (F) Representative current clamp recording from a MCH/CART− cell before (baseline) and during ZD 7288 application. (G) Repeated measures of voltage sag at baseline and in the presence of ZD 7288 in MCH/CART− cells. (H) X–Y scatter plot of the membrane potential at the peak of a voltage sag and ensuing steady-state in MCH/CART− cells shown in (G) . (I) Repeated measures of voltage sag at baseline and in the presence of ZD 7288 in MCH/CART+ cells. (J) Example recording of depolarized and hyperpolarized CART-positive MCH neurons. A minor population of MCH/CART+ neurons shows depolarized RMP like that of CART− neurons (>−70 mV) but lacks H-current. (K) A summary graph showing that H-current is unique to MCH/CART− neurons. (L,M) Steady-state potential in response to 600-ms hyperpolarizing current steps of varying magnitude in male (L) and female (M) MCH neurons. (C–E) $$ p < 0.01, $$$ p < 0.001, $$$$ p < 0.0001, LH CART− vs. all CART+ groups. (G,K) ** p < 0.01, *** p < 0.001, **** p < 0.0001. (L,M) $ p < 0.05, $$$$ p < 0.0001, LH CART− vs. ZI; # p < 0.05, ## p < 0.01, LH CART− vs. LH CART+; & p < 0.05, &&& p < 0.001, LH CART− vs. MH. Mean ± SEM are shown. For (G–K) , male and female cells were pooled since no sex differences were found in H-current density (E) .

    Article Snippet: To identify co-localization of CART in MCH neurons, brain slices were treated with a cocktail of goat anti-MCH antibody (1:500; sc14509, Santa Cruz Biotechnology Inc.) and rabbit anti-CART antibody (1:500; H-003-62, Phoenix Pharmaceuticals Inc.), followed by appropriate secondary antibodies and AMCA-streptavidin.

    Techniques: Membrane, Injection

    Immunohistochemical detection of CART co-expression in MCH neurons. (A) Double immunofluorescence of MCH and CART. Boxed areas in the left image show the ZI (i) , the LH (ii) , and the MH (iii) , enlarged in the insets (right). MCH neurons with co-localization of CART (filled arrowheads) are more common in the ZI and MH. MCH/CART− neurons (open arrowheads) are more frequently found in the LH. Scale bar: (Left) 1 mm, (Right, insets) 100 μm. (B,C) Representative images of tdTomato-expressing MCH neurons filled with biocytin during patch clamp recording, classified as CART-negative (B) or CART-positive (C) . Scale bar: 50 μm.

    Journal: Frontiers in Cellular Neuroscience

    Article Title: Electrophysiological properties of melanin-concentrating hormone neuron subpopulations defined by anatomical localization and CART expression

    doi: 10.3389/fncel.2024.1439752

    Figure Lengend Snippet: Immunohistochemical detection of CART co-expression in MCH neurons. (A) Double immunofluorescence of MCH and CART. Boxed areas in the left image show the ZI (i) , the LH (ii) , and the MH (iii) , enlarged in the insets (right). MCH neurons with co-localization of CART (filled arrowheads) are more common in the ZI and MH. MCH/CART− neurons (open arrowheads) are more frequently found in the LH. Scale bar: (Left) 1 mm, (Right, insets) 100 μm. (B,C) Representative images of tdTomato-expressing MCH neurons filled with biocytin during patch clamp recording, classified as CART-negative (B) or CART-positive (C) . Scale bar: 50 μm.

    Article Snippet: To identify co-localization of CART in MCH neurons, brain slices were treated with a cocktail of goat anti-MCH antibody (1:500; sc14509, Santa Cruz Biotechnology Inc.) and rabbit anti-CART antibody (1:500; H-003-62, Phoenix Pharmaceuticals Inc.), followed by appropriate secondary antibodies and AMCA-streptavidin.

    Techniques: Immunohistochemical staining, Expressing, Immunofluorescence, Patch Clamp

    Different Expression between AD and control groups of the five feature genes. (A) Differential expression of the five genes in merged GEO dataset. (A) Validation of the expression of the five genes (AEBP1, CARTPT, NPTX2, COPG2IT1, and AK5) by quantitative real-time reverse-transcription PCR (qRT-PCR) using brain tissues from AD mice and controls. (* p < 0.05, ** p < 0.01, *** p < 0.005, **** p < 0.0001). (C) Representative immunofluorescence images of AEBP1 in the cortices of AD and control group mice (scar bar: 20 μm). (D) Representative immunohistochemical images of CARTPT in the brains of AD and control group mice (scar bar: 50 μm).

    Journal: Heliyon

    Article Title: Identification and experimental validation of m7G-related molecular subtypes, immune signature, and feature genes in Alzheimer's disease

    doi: 10.1016/j.heliyon.2024.e33836

    Figure Lengend Snippet: Different Expression between AD and control groups of the five feature genes. (A) Differential expression of the five genes in merged GEO dataset. (A) Validation of the expression of the five genes (AEBP1, CARTPT, NPTX2, COPG2IT1, and AK5) by quantitative real-time reverse-transcription PCR (qRT-PCR) using brain tissues from AD mice and controls. (* p < 0.05, ** p < 0.01, *** p < 0.005, **** p < 0.0001). (C) Representative immunofluorescence images of AEBP1 in the cortices of AD and control group mice (scar bar: 20 μm). (D) Representative immunohistochemical images of CARTPT in the brains of AD and control group mice (scar bar: 50 μm).

    Article Snippet: Primary antibodies recognizing CARTPT (1:100, Abclonal, A18275) were used for the IHC staining.

    Techniques: Expressing, Control, Quantitative Proteomics, Biomarker Discovery, Reverse Transcription, Quantitative RT-PCR, Immunofluorescence, Immunohistochemical staining